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Protein expression of MBD1, MBD2, MeCP2, BRCA1, BRCA2 & p16 genes were done by western blotting in resveratrol treated MDA-MB-231, MCF-7, T-47D & MCF-10A breast normal and cancer cell lines. Bands were transferred to PVDF membrane and exposed to X-ray film. Densitometry analysis was done by <t>MyImage</t> analysis software (thermo Scientific) to quantify the bands intensity and normalized with housekeeping β-actin gene protein and bar graph plotted as fold change in protein expression vs. concentration of resveratrol
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Protein expression of MBD1, MBD2, MeCP2, BRCA1, BRCA2 & p16 genes were done by western blotting in resveratrol treated MDA-MB-231, MCF-7, T-47D & MCF-10A breast normal and cancer cell lines. Bands were transferred to PVDF membrane and exposed to X-ray film. Densitometry analysis was done by <t>MyImage</t> analysis software (thermo Scientific) to quantify the bands intensity and normalized with housekeeping β-actin gene protein and bar graph plotted as fold change in protein expression vs. concentration of resveratrol
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Protein expression of MBD1, MBD2, MeCP2, BRCA1, BRCA2 & p16 genes were done by western blotting in resveratrol treated MDA-MB-231, MCF-7, T-47D & MCF-10A breast normal and cancer cell lines. Bands were transferred to PVDF membrane and exposed to X-ray film. Densitometry analysis was done by MyImage analysis software (thermo Scientific) to quantify the bands intensity and normalized with housekeeping β-actin gene protein and bar graph plotted as fold change in protein expression vs. concentration of resveratrol

Journal: BMC Cancer

Article Title: Methyl CpG binding protein MBD2 has a regulatory role on the BRCA1 gene expression and its modulation by resveratrol in ER+, PR+ & triple-negative breast cancer cells

doi: 10.1186/s12885-024-12274-x

Figure Lengend Snippet: Protein expression of MBD1, MBD2, MeCP2, BRCA1, BRCA2 & p16 genes were done by western blotting in resveratrol treated MDA-MB-231, MCF-7, T-47D & MCF-10A breast normal and cancer cell lines. Bands were transferred to PVDF membrane and exposed to X-ray film. Densitometry analysis was done by MyImage analysis software (thermo Scientific) to quantify the bands intensity and normalized with housekeeping β-actin gene protein and bar graph plotted as fold change in protein expression vs. concentration of resveratrol

Article Snippet: Densitometry analysis was done by MyImage analysis software (thermo Scientific) to quantify the bands intensity and normalized with housekeeping β-actin gene protein and bar graph plotted as fold change in protein expression vs. concentration of resveratrol The association between protein expression and resveratrol levels was also investigated using correlation analysis.

Techniques: Expressing, Western Blot, Membrane, Software, Concentration Assay